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Inhibition of biofilm formation and swarming of Bacillus subtilis by (5 Z )‐4‐bromo‐5‐(bromomethylene)‐3‐butyl‐2(5 H )‐furanone
Author(s) -
Ren D.,
Sims J.J.,
Wood T.K.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01087.x
Subject(s) - bacillus subtilis , biofilm , swarming motility , swarming (honey bee) , motility , microbiology and biotechnology , bacteria , confocal laser scanning microscopy , biology , bacillales , bacillaceae , chemistry , quorum sensing , genetics
Aims: ( 5Z )‐4‐Bromo‐5‐(bromomethylene)‐3‐butyl‐2( 5H )‐furanone ( furanone) of the marine alga Delisea pulchra was synthesized, and its inhibition of swarming motility and biofilm formation of Bacillus subtilis was investigated. Methods and Results: Furanone was found to inhibit both the growth of B. subtilis and its swarming motility in a concentration‐dependent way. In addition, as shown by confocal scanning laser microscopy, furanone inhibited the biofilm formation of B. subtilis . At 40 μg ml −1 , furanone decreased the biofilm thickness by 25%, decreased the number of water channels, and reduced the percentage of live cells by 63%. Conclusions, Significance and Impact of the Study: Natural furanone has potential for controlling the multicellular behaviour of Gram‐positive bacteria.