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Improvement in the resting‐cell bioconversion of penicillin G to deacetoxycephalosporin G by addition of catalase
Author(s) -
Gao Q.,
Demain A.L.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01084.x
Subject(s) - bioconversion , penicillin , catalase , chemistry , economics , microbiology and biotechnology , biology , biochemistry , antibiotics , fermentation , enzyme
Aims: To improve the resting cell bioconversion of penicillin G to deacetoxycephalosporin G (DAOG) by elimination of an oxidizing intermediate which inactivates the enzyme during the reaction. Methods and Results: Resting cells of Streptomyces clavuligerus strain NP1 were incubated with penicillin G, required co‐factors and decane in the presence of catalase or superoxide dismutase, and production of DAOG was measured. Catalase stimulated the bioconversion but superoxide dismutase did not. Conclusions: Production of hydrogen peroxide during the ring expansion reaction is at least partially responsible for enzyme inactivation. Significance and Impact of the Study: Catalase addition improves the bioconversion and will contribute to the eventual replacement of the current multi‐step, expensive and environmentally‐unfriendly chemical ring expansion by a biological route.