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Expression and processing of a major xylanase (XYN2) from the thermophilic fungus Humicola grisea var. thermoidea in Trichoderma reesei
Author(s) -
De Faria F.P.,
Te'O V.S.J.,
Bergquist P.L.,
Azevedo M.O.,
Nevalainen K.M.H.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01057.x
Subject(s) - library science , computer science
Aims: To express a gene encoding a heterologous fungal xylanase in Trichoderma reesei . Methods and Results:Humicola grisea xylanase 2 ( xyn2 ) cDNA was expressed in Trichoderma reesei under the main cellobiohydrolase I ( cbh1 ) promoter (i) as a fusion to the cellobiohydrolase I (CBHI) secretion signal and (ii) the mature CBHI core‐linker. The recombinant xylanase (HXYN2) was secreted into the cultivation medium and processed in a similar fashion to the endogenous T. reesei xylanases, resulting in an active enzyme. Conclusions, Significance and Impact of the Study: HXYN2 was successfully processed in T. reesei . Composition of the culture medium affected the HXYN2 yields, favouring Avicel‐lactose as a carbon source. Best yields (about 0·5 g l −1 ) in shake flask cultivations were obtained from a transformant where xyn2 was fused directly to the CBHI secretion signal.