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Nisin and pediocin production on mussel‐processing waste supplemented with glucose and five nitrogen sources
Author(s) -
Guerra N.P.,
Pastrana L.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01054.x
Subject(s) - nisin , bacteriocin , yeast extract , mussel , food science , chemistry , biology , biochemistry , microbiology and biotechnology , fermentation , antimicrobial , fishery
Aims: Optimization of bacteriocin production by L. lactis subsp. lactis CECT 539 and Ped. acidilactici NRRL B‐5627 on mussel‐processing wastes. Methods and Results: The concentrations of glucose and five nitrogen sources that optimize nisin and pediocin production were determined using a second order orthogonal factorial design. NH 4 Cl, glycine and glutamic acid were poor nitrogen sources. Enhanced pediocin and nisin productions were achieved in a medium supplemented with yeast extract or Bacto casitone. Conclusions: Mussel‐processing wastes could be successfully used as a culture medium for bacteriocin production at low production cost. Significance and Impact of the Study: Average optimization led to a threefold increase in pediocin production (from 322 to 934 BU ml −1 ) and in nisin production (from 32 to 100 BU ml −1 ) when compared with the unsupplemented medium. For this reason, mussel‐processing waste warrants further investigation due to its potential use as a cheap culture medium for upscaling bacteriocin production.