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Development of membrane filter holder (MFH) method for recovery of heat‐injured Escherichia coli O157:H7 and Salmonella typhimurium
Author(s) -
Kang D.H.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01038.x
Subject(s) - membrane filter , salmonella , escherichia coli , microbiology and biotechnology , library science , biology , bacteria , membrane , computer science , biochemistry , genetics , gene
Aims: A method of recovering sublethally heat‐injured bacteria was developed with specific apparatus (membrane filter holder; MFH) which was originally used for Iso‐Grid Hydrophobic membrane TM . filter holder. Methods and Results: The procedure used a non‐selective agar underlayed with a selective medium with a MFH. A non‐selective agar was poured on upper part (compartment A) of MFH, and then injured foodborne pathogens were inoculated on the non‐selective medium. After 3‐h repair incubation period, selective agar was added to the bottom of the chamber (compartment B) of the MFH and further incubated. By diffusing through the non‐selective top agar, selective agents from the underlay medium impart selectivity to the system. Conclusions: Using the MFH method, recovery of heat‐injured foodborne pathogens (Escherichia coli O157:H7 and Salmonella typhimurium ) were not different ( P > 0·05) from recoveries with non‐selective media (TSA). However, the recoveries of foodborne pathogens on MFH were significantly higher ( P < 0·05) than those of direct plating on selective medium such as SMAC (MacConkey Sorbitol Agar) or XLD (Xylose Lysine Desoxycholate). Significance and Impact of the Study: In conclusion, the MFH method is a simple and convenient method for recovery of heat‐injured foodborne pathogens.