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A versatile reactor for continuous monitoring of biofilm properties in laboratory and industrial conditions
Author(s) -
Pereira M.O.,
Morin P.,
Vieira M.J.,
Melo L.F.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01030.x
Subject(s) - biofilm , contamination , flow conditions , flow (mathematics) , microbiology and biotechnology , environmental science , biochemical engineering , bacteria , biology , ecology , engineering , genetics , geometry , mathematics
Aims: The understanding of the dynamics of surface microbial colonization with concomitant monitoring of biofilm formation requires the development of biofilm reactors that enable direct and real‐time evaluation under different hydrodynamic conditions. Methods and Results: This work proposes and discusses a simple flow cell reactor that provides a means to monitoring biofilm growth by periodical removing biofilm‐attached slides for off‐line, both non‐destructive and destructive biofilm analyses. This is managed without the stoppage of the flow, thus reducing the contamination and the disturbance of the biofilm development. With this flow cell, biofilm growth and respiratory activity can be easily followed, either in well‐defined laboratory conditions or in an industrial environment. Conclusions, Significance and Impact of the Study: The reproducible and typical biofilm development curves obtained, validated this flow cell and confirmed its potential for different biofilm‐related studies, which can include biocidal treatment.