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A rapid and sensitive method for the detection of Yersinia enterocolitica strains from clinical samples
Author(s) -
Hussein H.M.,
Fenwick S.G.,
Lumsden J.S.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.01028.x
Subject(s) - yersinia enterocolitica , agar , microbiology and biotechnology , chromogenic , agar plate , plating (geology) , cefsulodin , enterobacteriaceae , bacteria , chromatography , chemistry , biology , escherichia coli , piperacillin , biochemistry , paleontology , genetics , gene , pseudomonas aeruginosa
Aims: To compare three culture methods to detect Yersinia enterocolitica from oral or rectal swabs from experimentally infected pigs. Methods and Results: The three methods used were: direct plating on Cefsulodin‐Irgasan‐Novobiocin (CIN) agar, cold enrichment in phosphate buffered saline (PBS) followed by plating on CIN agar and selective enrichment with Luria‐Bertani‐Bile Salts Irgasan (LB‐BSI) followed by plating on CIN agar. Selective enrichment with LB‐BSI produced the highest recovery rate (63%), when compared with cold enrichment (52%) and plating on CIN agar alone (43%). Selective enrichment with LB‐BSI was significantly ( P < 0·02) more sensitive than direct plating on CIN agar and more sensitive than cold enrichment ( P < 0·1). Conclusions, Significance and Impact of the Study: Selective enrichment with LB‐BSI was more sensitive than the widely accepted method of cold enrichment and it reduced the time required for detection of Y. enterocolitica by three weeks. Selective enrichment with LB‐BSI was also compatible with a multiplex PCR technique.