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Rapid identification of Lactobacillus brevis using the polymerase chain reaction
Author(s) -
Guarneri T.,
Rossetti L.,
Giraffa G.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.01014.x
Subject(s) - lactobacillus brevis , identification (biology) , polymerase chain reaction , classics , art , biology , ecology , bacteria , genetics , gene , lactic acid , lactobacillus plantarum
Aims: Species‐specific PCR was applied to identify Lactobacillus brevis and the sensitivity and the specificity of the protocol were determined. Methods and Results: Strains of Lact. brevis obtained from foods, particularly dairy products, and various strain collections, were identified by PCR using primers which amplified a 1340 bp fragment within the 16S rRNA gene. The PCR product was obtained after amplification of all the Lact. brevis strains tested; the size of the amplicon was as expected. No PCR products were observed after amplification from DNA of several lactic acid bacteria (LAB) species. Conclusions: A PCR method was optimized to identify Lact. brevis . The protocol was highly efficient and sensitive. Significance and Impact of the Study: Conventional phenotypic methods often lead to ambiguous identification of LAB species belonging to Lact. brevis . The proposed protocol is sensitive, specific, and can be applied to total DNA extracted by use of chelating matrix with loss of neither sensitivity nor specificity.