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A rapid, non‐destructive method for the determination of Staphylococcus epidermidis adhesion to surfaces using quartz crystal resonant sensor technology
Author(s) -
Pavey K.D.,
Barnes L.M.,
Hanlon G.W.,
Olliff C.J.,
Ali Z.,
Paul F.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.01009.x
Subject(s) - staphylococcus epidermidis , biofilm , adhesion , quartz , materials science , electrode , bacteria , chemistry , microbiology and biotechnology , staphylococcus aureus , composite material , biology , genetics
Aims: To investigate the use of quartz crystal resonant sensor (QCRS) technology to determine the adhesion of Staphylococcus epidermidis to fibronectin‐coated surfaces. Methods and Results: QCRS sensors (14 MHz) with 4 mm gold electrodes were coated with fibronectin and exposed for 15 min to suspensions of Staph. epidermidis ranging in concentration from 1 × 10 2 to 1 × 10 6 cfu ml −1 . Changes in resonant frequency were recorded and showed a linear relationship with the logarithm of cell concentration over the range tested. Conclusions: QCRS technology was shown to be a rapid, sensitive and non‐destructive method for measuring the adhesion of bacteria to surfaces. Significance and Impact of the Study: This report demonstrates that QCRS technology has the potential to be used for a range of applications requiring measurement of bacteria on surfaces. In particular, it may be used for the real‐time monitoring of bacterial biofilm formation.