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Differentiation of aflatoxin‐producing and non‐producing strains of Aspergillus flavus group
Author(s) -
Criseo G.,
Bagnara A.,
Bisignano G.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.00998.x
Subject(s) - aspergillus flavus , aflatoxin , biology , multiplex polymerase chain reaction , gene , microbiology and biotechnology , genetics , polymerase chain reaction
Aims: Three conventional methods and a multiplex PCR procedure with a set of four primers (Quadruplex‐PCR) were used to differentiate between aflatoxin‐producing and non‐producing strains of the Aspergillus flavus group. Methods and Results: By combining sets of primers for aflR , nor‐1 , ver‐1 and omt‐A genes of the aflatoxin biosynthetic pathway, Quadruplex‐PCR showed that aflatoxinogenic strains gave a quadruplet pattern, indicating the presence of all the genes involved in the aflatoxin biosynthetic pathway which encode for functional products. Non‐aflatoxinogenic strains gave varying results with one, two, three or four banding patterns. A banding pattern in three non‐aflatoxinogenic strains resulted in non‐differentiation between these and aflatoxinogenic strains. Conclusion and Significance and Impact of the Study: Because conventional methods are time‐consuming, further studies are needed to develop a rapid and objective technique that permits complete differentiation between aflatoxin‐producing and non‐producing strains of the A. flavus group.