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Polysaccharide production by a reduced pigmentation mutant of Aureobasidium pullulans NYS‐1
Author(s) -
West T.P.,
Strohfus B.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.00975.x
Subject(s) - aureobasidium pullulans , pullulan , polysaccharide , mutant , sucrose , food science , chemistry , carbon source , biomass (ecology) , strain (injury) , biochemistry , biology , microbiology and biotechnology , fermentation , gene , ecology , anatomy
Aims: To isolate a reduced pigmentation mutant of Aureobasidium pullulans NYS‐1 and characterize its cellular pigmentation plus its polysaccharide and biomass production relative to carbon source. Methods and Results: Cellular pigmentation, polysaccharide levels and biomass production by the isolated mutant NYSRP‐1 were analysed relative to carbon source. Cellular pigmentation of the mutant was lower than its parent strain using either carbon source. The mutant elaborated higher polysaccharide levels on sucrose than on corn syrup. The pullulan content of the polysaccharide synthesized and biomass production by the mutant rose as the carbon source concentration was increased. Conclusions: It is feasible to isolate a reduced pigmentation mutant from strain NYS‐1 that exhibits elevated polysaccharide production using corn syrup as a carbon source. Significance and Impact of the Study: The mutant provides an advantage for commercial pullulan production because of its reduced pigmentation and enhanced polysaccharide synthesis.