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Characterization of pectin lyase produced by an endophytic strain isolated from coffee cherries
Author(s) -
Sakiyama C.C.H.,
Paula E.M.,
Pereira P.C.,
Borges A.C.,
Silva D.O.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.00961.x
Subject(s) - pectin lyase , pectinase , bacteria , pectin , food science , plant use of endophytic fungi in defense , strain (injury) , chemistry , enzyme , biology , extremophile , botany , microbiology and biotechnology , biochemistry , microorganism , anatomy , genetics
Aims: The effect of endophytic bacterial activity on the quality of coffee beverage was studied. Methods and Results: A survey of the micro‐organisms in coffee cherries was performed before harvesting, and their growth on the main nutrients available in coffee cherries was determined in vitro . Conclusions: Many endophytic bacteria were isolated from surface‐sterilized coffee cherries. One of the pectinolytic strains was physiologically and phenotypically characterized, and was tentatively identified by partial 16S rDNA sequencing as Paenibacillus amylolyticus . This endophytic strain produced an extracellular pectinase with maximal activity at 40°C and pH 7·9, and was thermostable up to 45°C. EDTA and metal ions had little effect on pectin lyase activity. K m and V max values were 4·6 mg ml –1 and 94·0 10 –8 mol min –1 ml –1 , respectively. Significance and Impact of the Study: Pectin lyases have been found in fungi but rarely in bacteria, and this isolate is a promising tool for regulation studies of these enzymes.