Premium
Improved acid tolerance of a recombinant strain of Escherichia coli expressing genes from the acidophilic bacterium Oenococcus oeni
Author(s) -
Morel F.,
Delmas F.,
Jobin M.P.,
Diviès C.,
Guzzo J.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.00960.x
Subject(s) - oenococcus oeni , biology , escherichia coli , heterologous expression , gene , open reading frame , microbiology and biotechnology , genetics , plasmid , bacteria , recombinant dna , peptide sequence , malolactic fermentation , lactic acid
Aims:Oenococcus oeni is a lactic acid bacterium used in wine fermentation. Two open reading frames ( orfB and orfC ) were identified in the upstream region of the hsp18 gene, encoding the small heat‐shock protein Lo18. Expression of these genes in conditions of acid stress was studied in Escherichia coli . Methods and Results: Sequence analysis showed that orfB encodes a putative transcriptional regulator of the LysR family. The protein encoded by orfC shares homologies with multi‐drug resistance systems. Heterologous expression of orfB , orfC and hsp18 genes in Escherichia coli significantly enhanced the viability of the host strain under acidic conditions. Conclusions: It was demonstrated that the three genes were needed for acquisition of this acid tolerance phenotype. Significance and Impact of the Study: Heterologous expression of Oenococcus genes could be used to confer acidophilic behaviour on strains of biotechnological interest.