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Optimization of submerged culture conditions for the mycelial growth and exo‐biopolymer production by Cordyceps militaris
Author(s) -
Park J.P.,
Kim S.W.,
Hwang H.J.,
Yun J.W.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.00950.x
Subject(s) - biopolymer , cordyceps militaris , mycelium , sucrose , industrial fermentation , food science , fermentation , nitrogen , chemistry , botany , biology , cordyceps , polymer , organic chemistry
Aims: The objective of the present study was to determine the optimal culture conditions for exo‐biopolymer production by Cordyceps militaris in shake flask culture. Methods and Results: The optimal temperature and initial pH for both mycelial growth and exo‐biopolymer production by Cordyceps militaris in shake flask culture were found to be 20°C and 6·0, respectively. Sucrose (40 g l –1 ) and corn steep powder (10 g l –1 ) were the most suitable carbon and nitrogen source for both mycelial growth and exo‐biopolymer production. Conclusions: Under optimal culture conditions, the maximum exo‐biopolymer concentration in a 5‐l jar fermenter indicated 10·3 g l –1 , which was approximately three times higher than that in shake flask culture. Significance and Impact of the Study: This process can have a significant impact on the industrial scale when sucrose and corn steep powder were used as carbon and nitrogen source.