Premium
Differentiation of Enterococcus spp. by cell membrane fatty acid methyl ester profiling, biotyping and ribotyping
Author(s) -
Lang M.M.,
Ingham S.C.,
Ingham B.H.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.00949.x
Subject(s) - ribotyping , biology , ecori , enterococcus , microbiology and biotechnology , enterococcus faecalis , ribosomal rna , dna profiling , bacteria , restriction enzyme , dna , biochemistry , genetics , gene , polymerase chain reaction , staphylococcus aureus
Aims: Gas chromatographic analysis of cell membrane fatty acid methyl esters (FAME), biochemical profiling (biotyping) and Eco RI restriction endonuclease profiling of DNA containing ribosomal RNA sequences (ribotyping) were compared for differentiation of Enterococcus spp. Methods and Results: FAME profiling, biotype profiling and ribotyping of 41 strains from retail Swiss‐type cheeses and five strains from culture collections resulted in 17, 25 and 26 groups, respectively, with only two pairs of strains having the same FAME group, biotype profile and ribogroup. Conclusions: Substantial overlap occurred in groupings assigned by the three methods. Significance and Impact of the Study: Differentiation of Enterococcus spp. strains increases if multiple methods are used.