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Optimization of carotenoid production from hyper‐producing Rhodotorula glutinis mutant 32 by a factorial approach
Author(s) -
Bhosale P.,
Gadre R.V.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.00940.x
Subject(s) - rhodotorula , division (mathematics) , library science , microbiology and biotechnology , chemistry , biology , mathematics , computer science , yeast , biochemistry , arithmetic
Aims: Optimization of carotenoid production by a mutant of Rhodotorula glutinis . Methods: The growth and carotenoid production was optimized in shake flasks using a two‐level, three‐variable factorial design. Results: The volumetric carotenoid production could be increased to 129 ± 2 mg 1 −1 in a medium containing (g l −1 ) yeast extract 11·74, glucose 46 and threonine 18 along with other micronutrients, wherein, β ‐carotene yield was 102 ± 2 mg l −1 , accounting for 80% of the total carotenoids. Significance and Impact of the Study: The medium optimization resulted in a fourfold increase in volumetric production and a twofold increase in the cellular accumulation of carotenoids. In view of such high yields, the mutant of Rhodotorula glutinis can be a potential source of β ‐carotene.