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A microwave‐based method for nucleic acid isolation from environmental samples
Author(s) -
Orsini M.,
RomanoSpica V.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.00938.x
Subject(s) - isolation (microbiology) , dna extraction , dna , nucleic acid , microwave , reagent , biochemical engineering , microwave heating , computational biology , environmental science , chromatography , biology , computer science , chemistry , polymerase chain reaction , microbiology and biotechnology , biochemistry , engineering , telecommunications , gene
Aims: A simple and rapid method was described for DNA isolation directly from activated sludge or other environmental sources, including soil and sediments. Methods and Results: The present method is based on microwave thermal shock and provides DNA suitable for further analysis. It is also effective for RNA extraction. Conclusions: The protocol is effective, easy, fast and does not require the use of expensive equipment or reagents. Significance and Impact of the Study: The described method can be applied to difficult substrates in environmental microbiology studies.