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Tolerance of Bradyrhizobium sp. ( Lupini ) strains to salinity, pH, CaCO 3 and antibiotics
Author(s) -
Raza S.,
Jørnsgård B.,
AbouTaleb H.,
Christiansen J.L.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.00925.x
Subject(s) - microbial inoculant , bradyrhizobium , salinity , antibiotics , strain (injury) , biology , microbiology and biotechnology , food science , bacteria , chemistry , veterinary medicine , zoology , nitrogen fixation , ecology , genetics , anatomy , medicine
Aims: Ten rhizobial isolates obtained from different locations in Egypt were examined for their ability to survive under stress conditions and their growth response to increasing levels of NaCl (1–8% w/v), pH (4–10), CaCO 3 (1–10% w/v) and 12 antibiotics. Methods and Results: All the rhizobial isolates tolerated a NaCl concentration up to 5% and were divided into two groups with respect to NaCl tolerance. The rhizobial isolates from group two showed significantly ( P < 0·05) better survival under high NaCl concentration. All the tested isolates survived acidic (pH 4–5) and alkaline conditions (pH 9–10) and CaCO 3 (up to 10% w/v) in liqued YEM medium. Conclusions: Antibiotic resistance patterns did not correlate to NaCl, pH or CaCO 3 tolerance. Variations among different strains showed that there is potential to improve strain performance under stress conditions. Significance and Impact of the Study: The results suggest that selection of adapted strains under stress conditions is possible and can be used as inoculants for successful lupin growth.