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Temporal temperature gradient gel electrophoresis (TTGE) as a tool for identification of Lactobacillus casei , Lactobacillus paracasei , Lactobacillus zeae and Lactobacillus rhamnosus
Author(s) -
Vásquez A.,
Ahrné S.,
Pettersson B.,
Molin G.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.00901.x
Subject(s) - lactobacillus casei , lactobacillus paracasei , lactobacillus , biology , lactobacillus rhamnosus , 16s ribosomal rna , microbiology and biotechnology , temperature gradient gel electrophoresis , bacteria , genetics
Aims: To develop a tool for rapid and inexpensive identification of the Lactobacillus casei complex. Methods and Results:Lactobacillus casei , Lactobacillus paracasei , Lactobacillus zeae and Lactobacillus rhamnosus were identified by PCR‐amplification of the segment between the U1 and U2 regions of 16S rDNA (position 8–357, Escherichia coli numbering) and temporal temperature gradient gel electrophoresis (TTGE). Seven tested Lact. paracasei strains were divided into three TTGE‐subgroups. Conclusions: TTGE successfully distinguished between the closely‐related target species. TTGE is also a powerful method for revealing sequence heterogeneities in the 16S rRNA genes. Significance and Impact of the Study: Due to rapid and easy performance, TTGE of PCR‐amplified 16S rDNA fragments will be useful for the identification of extended numbers of isolates.