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Detection of tabtoxin‐producing strains of Pseudomonas syringae by PCR
Author(s) -
Lydon J.,
Patterson C.D.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2001.00882.x
Subject(s) - pseudomonas syringae , microbiology and biotechnology , biology , pseudomonadaceae , pseudomonas , pseudomonadales , bacteria , polymerase chain reaction , virology , pathogen , genetics , gene
J. LYDON AND C. D. PATTERSON. 2001.Aims: The present study describes a system based on PCR to distinguish tabtoxin‐producing strains of Pseudomonas syringae from other Ps. syringae plant pathogens that produce chlorosis‐inducing phytotoxins. Methods and Results: Thirty‐two strains of Ps. syringae and related species were examined. Two sets of PCR primers were developed to amplify genes ( tblA and tabA ) required for tabtoxin production. Only a PCR product of 829 bp or 1020 bp was produced in PCR reactions with the tblA or tabA primer sets, respectively, and cells from tabtoxin‐producing pathovars of Pseudomonas syringae . All known non‐tabtoxin producing bacterial species failed to produce an amplification product with either primer set. Conclusions: PCR of genes required for tabtoxin production is a simple, rapid and reliable method for identifying tabtoxin‐producing strains of Ps. syringae . Significance and Impact of the Study: The protocol can effectively distinguish tabtoxin‐producing strains of Ps. syringae from other Ps. syringae pathovars and Ps. syringae pv. tabaci strains from other tabtoxin‐producing Ps. syringae pathovars.