Premium
Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR
Author(s) -
Civilini M.,
Pucillo C.,
Colombatti A.,
Damante G.,
De Bertoldi M.,
Tell G.
Publication year - 2000
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2000.00819.x
Subject(s) - monoclonal antibody , pseudomonadaceae , pseudomonas aeruginosa , pseudomonas , western blot , pseudomonadales , chemistry , microbiology and biotechnology , antibody , biology , blot , dioxygenase , naphthalene , enzyme , bacteria , biochemistry , gene , immunology , genetics , organic chemistry
A monoclonal antibody, designated mAbα(CT), was generated against a peptide of the ISP NAP α‐subunit of the naphthalene dioxygenase (NDO) enzyme of Pseudomonas aeruginosa . Since NDO expression is induced by aromatic hydrocarbons, its detection is important as a tool for environmental biomonitoring. This antibody is highly specific and works well both in an indirect ELISA assay and Western Blot analysis, allowing the detection of Pseudomonas spp. expressing the NDO inducible enzyme. The detection threshold for the ELISA assay developed in this work was 10 4 colony forming units (cfu) per ml. Thus, this mAb could represent a powerful tool to test for pollutants in soil, groundwater, and other natural environments.