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Use of the indirect immunofluorescence method for detection and enumeration of Escherichia coli in seawater samples
Author(s) -
Caruso G.,
Zaccone R.,
Crisafi E.
Publication year - 2000
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2000.00810.x
Subject(s) - enumeration , seawater , escherichia coli , immunofluorescence , detection limit , agar , biology , microbiology and biotechnology , indirect immunofluorescence , enterobacteriaceae , limit (mathematics) , chromatography , chemistry , bacteria , antigen , antibody , mathematics , ecology , biochemistry , immunology , mathematical analysis , genetics , combinatorics , gene
The determination of Escherichia coli in marine waters through a rapid method, the microscopic indirect immunofluorescent technique, is evaluated in comparison with the conventional count on m‐FC agar medium. The data obtained in seawater samples, collected monthly along the Messina coastline, show good sensitivity of the analysis and agreement between the microscopic and culture technique, with a detection limit of 10 2 cells 100 ml −1 for immunofluorescence.