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The differentiation of Carnobacterium divergens using the random amplification of polymorphic DNA polymerase chain reaction technique
Author(s) -
Lai S.,
Shojaei H.,
Manchester L.N.
Publication year - 2000
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2000.00745.x
Subject(s) - polymerase chain reaction , biology , dna , multiple displacement amplification , microbiology and biotechnology , genetics , dna extraction , gene
The potential of the randomly amplified polymorphic DNA polymerase chain reaction (RAPD‐PCR) technique to differentiate Carnobacterium divergens from other members of the genus Carnobacterium was examined. A numerical analysis of the genomic profiles obtained demonstrated that it was possible to differentiate the C. divergens strains from other Carnobacterium strains using this technique. The heterogeneity observed in the representatives of the species C. piscicola adds further weight to the suggestion in other taxonomic studies that subspecies of this species exist.