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RAPD‐fingerprinting of Serratia marcescens after formaldehyde inactivation of DNase activity
Author(s) -
Polyzou A.,
Sofianou D.,
Pournaras S.,
Tsakris A.
Publication year - 2000
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2000.00743.x
Subject(s) - serratia marcescens , rapd , dna profiling , biology , microbiology and biotechnology , dna , dna extraction , polymerase chain reaction , genetics , escherichia coli , genetic diversity , gene , medicine , population , environmental health
Serratia marcescens strains commonly cause hospital outbreaks. A random amplified polymorphic DNA (RAPD) assay was applied to the epidemiology of Ser. marcescens using formaldehyde fixation and boiling of bacterial cells for DNA extraction. The method preserved bacterial DNA and gave optimal results of RAPD‐fingerprinting, facilitating the investigation of hospital infections caused by Ser. marcescens .