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Cytotoxicity of alkaline‐tolerant dairy‐associated Bacillus spp.
Author(s) -
Lindsay D.,
Mosupye F.M.,
Brözel V.S.,
Von Holy A.
Publication year - 2000
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2000.00732.x
Subject(s) - propidium iodide , bacillus pumilus , cytotoxicity , cytotoxic t cell , bacillus licheniformis , staining , biology , microbiology and biotechnology , mtt assay , chemistry , bacteria , biochemistry , cell , in vitro , bacillus subtilis , apoptosis , programmed cell death , genetics
The cytotoxicity of five Bacillus spp. isolated from alkaline cleaning solutions in South African dairies was evaluated against McCoy mouse cells using a 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT)‐based assay, confocal scanning laser microscopy and scanning electron microscopy. According to the MTT‐based assay, two of the Bacillus isolates ( Bacillus licheniformis 5 and B. pumilus 122) were cytotoxic to McCoy cells and the cytotoxic components were heat labile. Confocal scanning laser microscopy combined with fluorescent staining using propidium iodide and fluorescein diacetate indicated that cytotoxic effects occurred within 3 h, appeared to be membrane active and resulted in cell necrosis. Scanning electron microscopy showed that McCoy cells exposed to the cytotoxic components exhibited morphological damage.