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Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity
Author(s) -
Nostro A.,
Germanò M.P.,
D’Angelo V.,
Marino A.,
Cannatelli M.A.
Publication year - 2000
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2000.00731.x
Subject(s) - antimicrobial , phytochemical , traditional medicine , agar diffusion test , minimum inhibitory concentration , terpene , medicinal plants , biology , gram , thin layer chromatography , gram negative bacteria , bacteria , antibacterial activity , chemistry , botany , microbiology and biotechnology , chromatography , escherichia coli , biochemistry , medicine , genetics , gene
A comparative study on the antimicrobial properties of extracts from medicinal plants obtained by two different methods was carried out. The screening of the antimicrobial activity of extracts from six plants was conducted by a disc diffusion test against Gram‐positive, ‐negative and fungal organisms. The most active extracts (inhibition diameter ≥12 mm) were assayed for the minimum inhibitory concentration and submitted to phytochemical screening by thin‐layer chromatography and bioautography. The results obtained indicate that the diethyl ether extracts were the most efficient antimicrobial compounds. The activity was more pronounced against Gram‐positive and fungal organisms than against Gram‐negative bacteria. Bioautography showed that the antimicrobial activity was probably due to flavonoids and terpenes.