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Cluster analysis of AP‐PCR generated DNA fingerprints of Vibrio vulnificus isolates from patients fatally infected after consumption of raw oysters
Author(s) -
Vickery M.C.L.,
Harold N.,
Bej A.K.
Publication year - 2000
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2000.00707.x
Subject(s) - vibrio vulnificus , biology , polymerase chain reaction , genomic dna , dna profiling , microbiology and biotechnology , vibrionaceae , vibrio , genome , oyster , strain (injury) , dna , vibrio infections , genetics , gene , bacteria , anatomy , fishery
Arbitrarily‐primed‐polymerase chain reaction (AP‐PCR) DNA fingerprints were generated for 10 Vibrio vulnificus strains isolated from patients who became infected and died between 1993 and 1996 as a result of consuming raw oysters. Analysis of the DNA fingerprints with gel imaging and cluster analysis software revealed significant genetic heterogeneity among these strains, suggesting that V. vulnificus has a high degree of variation in its genomic organization, and that multiple pathogenic strains with greatly diverse genomic arrangements, rather than a single type of infective strain or serogroup, caused these infections.