Premium
A rapid biochemical test to aid identification of Mycoplasma mycoides subsp. mycoides small colony (SC) strains
Author(s) -
Rice P.,
Houshaymi B. M.,
Nicholas R. A. J.,
Miles R. J.
Publication year - 2000
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2000.00674.x
Subject(s) - mycoplasma mycoides , biology , microbiology and biotechnology , maltose , agar , mycoplasma , bacteria , biochemistry , enzyme , genetics
P. RICE, B.M. HOUSHAYMI, R.A.J. NICHOLAS and R.J. MILES.2000.The ability to utilize maltose, as determined by measurement of oxygen uptake, is used to differentiate Mycoplasma mycoides subsp. mycoides small colony (SC) and M. capricolum subsp. capripneumoniae (all strains negative) from other members of the M. mycoides cluster ( M. mycoides subsp. capri, M. mycoides subsp. mycoides large colony (LC), M. capricolum subsp. capricolum; and bovine serogroup 7; 94% of strains positive). Rapid tests for maltose utilizing ability were developed, based on hydrolysis of a chromogenic α‐glucosidase (maltase) substrate ( p ‐nitrophenyl‐α‐ d ‐glucopyranoside, colourless) to give a brightly coloured product ( p ‐nitrophenol, yellow). On agar plates, colonies of maltose‐utilizing strains became coloured within 40 min.