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Cloning and sequencing of ββ‐1,4‐endoglucanase gene ( celA ) from Pseudomonas sp. YD‐15
Author(s) -
Her S.,
Lee H. S.,
Choi S. J.,
Choi S. W.,
Choi H. J.,
Yoon S. S.,
Oh D. H.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1999.00651.x
Subject(s) - open reading frame , cellobiose , nucleic acid sequence , escherichia coli , molecular cloning , microbiology and biotechnology , gene , amino acid , biology , pseudomonas , biochemistry , homology (biology) , sequence analysis , peptide sequence , cellulase , genetics , bacteria , enzyme
A β‐1,4‐endoglucanase gene ( cel A) from Pseudomonas sp. YD‐15 was cloned in Escherichia coli DH5α and its nucleotide sequence determined. The open reading frame of celA was 1830 base pairs and the enzyme was composed of 609 amino acids with a molecular weight of 63 617 Da. The deduced amino acid sequence and putative active site of CelA had high amino acid homology with family E cellulases. By dot blot analysis, the induction of celA according to carbon sources was determined. The transcripts hybridizing to the internal fragment of celA were detected in total RNA isolated from Pseudomonas sp. YD‐15 cells grown on avicel and glycerol, but not from cells grown on glucose and cellobiose.