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Evaluation of two media for the membrane filtration enumeration of Clostridium perfringens from water
Author(s) -
Sartory D. P.,
Field M.,
Curbishley S. M.,
Pritchard A. M.
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00454.x
Subject(s) - clostridium perfringens , enumeration , spore , microbiology and biotechnology , agar , clostridiaceae , filtration (mathematics) , biology , chemistry , food science , veterinary medicine , bacteria , toxin , medicine , mathematics , statistics , genetics , combinatorics
Two media (mCP medium and Tryptose Sulphite Cycloserine (TSC) agar) were evaluated for recovery of Clostridium perfringens in environmental and part‐treated drinking water. For laboratory strains of Clostridium , mCP was more selective and specific for Cl. perfringens than TSC, but was markedly less efficient for the enumeration of both vegetative cells and spores. For samples of river water and part‐treated drinking water, TSC recovered significantly greater numbers of Cl. perfringens than mCP. In contrast to previous reports, there was a significant number of false presumptive positive and negative isolates on mCP. TSC is a more suitable medium for the routine monitoring of water supplies for the presence of Cl. perfringens .