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PCR differentiation of Escherichia coli from other Gram‐negative bacteria using primers derived from the nucleotide sequences flanking the gene encoding the universal stress protein
Author(s) -
Chen J.,
Griffiths M. W.
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00445.x
Subject(s) - escherichia coli , biology , gene , bacteria , genetics , nucleic acid sequence , enterobacteriaceae , nucleotide , polymerase chain reaction , gram negative bacteria , microbiology and biotechnology
A PCR‐based test was developed to differentiate Escherichia coli from other Gram‐negative bacteria. The assay employed primers derived from the nucleotide sequences flanking the gene encoding the universal stress protein ( uspA ) and was found to be highly specific for E. coli . An 884 base pair (bp) specific product was amplified from all of the E. coli tested ( n = 45), whereas no amplification was associated with non‐ E. coli Gram‐negative bacteria ( n = 11).