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Trichoderma /pathogen interactions: measurement of antagonistic chemicals produced at the antagonist/pathogen interface using a tubular bioassay
Author(s) -
Cooney J. M.,
Lauren D. R.
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00437.x
Subject(s) - botrytis cinerea , pathogen , bioassay , microbiology and biotechnology , biology , trichoderma , fungal pathogen , biotransformation , metabolite , fungi imperfecti , antifungal , secondary metabolite , botany , biochemistry , ecology , gene , enzyme
A tubular bioassay was used to measure analytically the local production and concentration of the antifungal Trichoderma secondary metabolite 6‐ n ‐pentyl‐ 2H ‐pyran‐2‐one (6PAP) at the Trichoderma antagonist/pathogen interface. 6PAP levels significantly increased in the presence of the pathogen Botrytis cinerea , typically 300–700%, and were highest near the pathogen source. The level of response for a particular Trichoderma isolate was found to vary with the test organism used. Two products produced by biotransformation of 6PAP by B. cinerea in response to the interaction were also detected.