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A RAPD‐PCR method for large‐scale typing of Bacillus cereus
Author(s) -
Nilsson J.,
Svensson B.,
Ekelund K.,
Christiansson A.
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00402.x
Subject(s) - rapd , bacillus cereus , typing , biology , microbiology and biotechnology , bacillaceae , polymerase chain reaction , genetics , bacteria , gene , bacillus subtilis , medicine , population , genetic diversity , environmental health
A robust RAPD-PCR procedure for large-scale typing of Bacillus cereus was developed. It is based on a simple DNA preparation, involving only freezing and boiling of cells in water with active carbon. By using a computerized system for data collection and processing, an efficient system for handling RAPD patterns for large-scale investigations was achieved. The procedure was highly discriminatory for Bacillus cereus strains and was found to give reproducible classification of RAPD fingerprints for five reference strains.