Premium
Escherichia coli O157 serology: false‐positive ELISA results caused by human antibodies binding to bovine serum albumin
Author(s) -
Chart H.,
Evans J.,
Chalmers R.M.,
Salmon R.L.
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00396.x
Subject(s) - escherichia coli , bovine serum albumin , serology , antibody , lipopolysaccharide , enterobacteriaceae , serum albumin , biology , microbiology and biotechnology , chemistry , immunology , biochemistry , gene
An analysis of farm workers and rural dwellers for serum antibodies to the lipopolysaccharide (LPS) of Escherichia coli O157 detected sera with antibodies binding to bovine serum albumin (BSA) by ELISA. These antibodies were not specific for BSA when examined by immunoblotting, and the ELISA values were reduced to a background level when plates were blocked with normal rabbit serum.