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Rapid purification of DNA from soil for molecular biodiversity analysis
Author(s) -
Yeates,
Michael R. Gillings
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00383.x
Subject(s) - dna , polymerase chain reaction , lysis , biology , dna extraction , ribosomal rna , microbiology and biotechnology , ribosomal dna , gene , genetics , phylogenetics
A rapid DNA extraction method utilizing a bead‐beating machine is described. High molecular weight DNA could be extracted from up to 24 samples in less than 2 h. The DNA was suitable for direct use in the polymerase chain reaction (PCR). Both prokaryotic and eukaryotic cells were successfully lysed by the method, established using primers specific for these groups. The small subunits ribosomal RNA (rRNA) spacer region from both eukaryotes and eubacteria could be readily amplified with DNA from different soils generating different amplification patterns.