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A PCR‐ELISA for the detection of potential fumonisin producing Fusarium species
Author(s) -
Christoph Grimm,
Geisen
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00366.x
Subject(s) - fusarium , fumonisin , biology , fumonisin b1 , ribosomal dna , ribosomal rna , mycotoxin , sequence analysis , botany , microbiology and biotechnology , phylogenetics , genetics , gene
A PCR‐ELISA for the detection of potential fumonisin producing Fusarium species has been developed, using the ribosomal ITS1 sequence as target. For this purpose, the sequences of the ITS1 regions of different fumonisin producing Fusarium species have been determined and compared to the sequences of fumonisin non‐producing species. In general, the ITS1 sequences were highly homologous. However, some minor sequence polymorphisms were detected, which differentiates potential fumonisin producing Fusarium species from non‐producing species. By using these sequence differences, a PCR‐ELISA for potential fumonisin producing Fusarium species was developed. All other ubiquitously occurring food‐borne fungi tested showed negative results with this test.