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Density of lectin‐like receptors in the FLO1 phenotype of Saccharomyces cerevisiae
Author(s) -
Patelakis,
Ritcey,
Speers
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00328.x
Subject(s) - saccharomyces cerevisiae , phenotype , lectin , receptor , biology , genetics , gene , biochemistry
An improved lectin assay was recently developed to determine the density of lectin sites present on the surface of a haploid strain 1209 of Saccharomyces cerevisiae exhibiting the Flo1 phenotype. The assay is based on the use of FITC‐avidin which contains a mannose side chain which functions as a binding site to the cell wall lectins termed zymolectins. An examination of the effect of culture time on the zymolectin density indicated no significant effect ( P > 0·05) confirming other findings that Flo1 acts in a constitutive manner. Zymolectin densities for this strain averaged 5·4 × 10 6 sites cell −1 .