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Inhibition effect of enterocin CCM 4231 in the rumen fluid environment
Author(s) -
Lauková,
Czikková
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00326.x
Subject(s) - enterococcus faecium , bacteriocin , listeria monocytogenes , streptococcus bovis , rumen , microbiology and biotechnology , colony forming unit , escherichia coli , strain (injury) , antimicrobial , fermentation , streptococcus , biology , bacteria , nisin , food science , chemistry , biochemistry , antibiotics , genetics , anatomy , gene
Enterocin CCM 4231 is a bacteriocin with a broad antimicrobial spectrum produced by the ruminal strain Enterococcus faecium CCM 4231. Its inhibitory effect towards enterococci, Ent. faecium EF 26/42, staphylococci, Streptococcus bovis AO 24/85 and Escherichia coli , as well as towards Listeria monocytogenes OHIO strain, in the rumen fluid environment was studied during culture at 37 and 30 °C for 24 h and 20 days. Enterocin CCM 4231 was added to the samples at a concentration of 3200 AU ml −1 . The best inhibitory effect was noted against enterococci at both cultivation temperatures. A decrease in total cell count from 10 8 cfu ml −1 to 10 4 cfu ml −1 and from 10 5 cfu ml −1 to 10 1 cfu ml −1 was detected. Addition of enterocin to the rumen fluid also inhibited staphylococci (from 10 5 cfu ml −1 to 10 4 cfu ml −1 and/or 10 3 cfu ml −1 ). Gram‐negative E. coli cells were inhibited at both cultivation temperatures (decrease from 10 6 cfu ml −1 to 10 1 cfu ml −1 at 37 °C, and from 10 7 cfu ml −1 to 10 5 cfu ml −1 at 30 °C). Enterococcus faecium EF 26/42 and Streptococcus bovis AO 24/85, the strains growing in the rumen fluid, were the most sensitive to the addition of enterocin during the first 24 h of fermentation (decrease from 10 10 cfu ml −1 and 10 8 cfu ml −1 to 10 6 cfu ml −1 and 10 4 cfu ml −1 ). An antilisterial effect of the bacteriocin was also confirmed. Further application of bacteriocin in ruminal ecology was indicated.