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Typing of Bacillus sporothermodurans and other Bacillus species isolated from milk by repetitive element sequence based PCR
Author(s) -
Herman Herman,
Heyndrickx,
Waes
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00314.x
Subject(s) - bacillaceae , biology , typing , microbiology and biotechnology , bacillales , bacillus (shape) , repeated sequence , bacteria , multilocus sequence typing , sequence (biology) , polymerase chain reaction , genetics , genotype , gene , bacillus subtilis , genome
When analysing raw milk for the presence of Bacillus sporothermodurans, 11 Bacillus strains were isolated which could be differentiated from known Bacillus, Brevibacillus and Paenibacillus spp. by different primer specificity in PCR experiments, indicating that they probably belong to Bacillus species as yet undescribed. Using repetitive element sequence based PCR (REP‐PCR), these 11 strains could be clearly distinguished from B. sporothermodurans as well as from each other. Eighty‐five B. sporothermodurans strains were characterized by a typical REP‐pattern. Using REP‐PCR combined with separation on non‐denaturing polyacrylamide gel electrophoresis and silver staining, individual B. sporothermodurans strains could be discriminated, which was not possible by methods previously published.