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Premium Improved cloning vectors for Bifidobacterium spp.
Publication year1998
Publication title
letters in applied microbiology
Resource typeJournals
PublisherBlackwell Science Ltd
The recombinant plasmids pDLI41, pDGA7 and pDCO7 were constructed by cloning in pDG7, a vector based on Bifidobacterium longum replicon pMB1, the following heterologous genes: Pseudomonas fluorescens lipase, Bacillus licheniformis α‐amylase and Streptomyces sp. cholesterol oxidase. The hybrid plasmids efficiently transformed Bifidobacterium belonging to five different species. A novel Escherichia coli‐Bifidobacterium set of shuttle vectors based on the replicon pMB1 (pLF5, pCLJ15, pSPEC1) featuring chloramphenicol, erythromycin and spectinomycin resistance genetic determinants as selection marker for bifidobacteria, was developed. The plasmid pTRE3, a derivative of pLF5, was the smallest (2·8 kb) Bifidobacterium vector, possessed a convenient multicloning site and presented high structural and segregational stability.
Subject(s)antibiotic resistance , antibiotics , bacillus licheniformis , bacillus subtilis , bacteria , bifidobacterium , bifidobacterium longum , biology , cloning vector , gene , genetics , lactobacillus , microbiology and biotechnology , multiple cloning site , plasmid , recombinant dna , replicon , shuttle vector , spectinomycin , vector (molecular biology)
Language(s)English
SCImago Journal Rank0.698
H-Index110
eISSN1472-765X
pISSN0266-8254
DOI10.1046/j.1472-765x.1998.00285.x

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