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Separation of Listeria monocytogenes and Salmonella berta from a complex food matrix by aqueous polymer two‐phase partitioning
Author(s) -
Bente Klarlund Pedersen,
Skouboe,
Rossen,
Rasmussen
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00275.x
Subject(s) - polyethylene glycol , listeria monocytogenes , dextran , chemistry , bacteria , salmonella , polyethylene , starch , aqueous solution , matrix (chemical analysis) , phase (matter) , polymer , chromatography , microbiology and biotechnology , food science , biology , organic chemistry , genetics
In the present study, the use of aqueous polymer two‐phase systems for separation of pathogenic bacteria from a complex food sample was investigated. Three different two‐phase systems, a polyethylene glycol 3350/dextran T 500, a methoxy polyethylene glycol 5000/dextran T 500 and a polyethylene glycol 3350/hydroxypropyl starch system, were compared at pH 3 and pH 6 for their capacity to separate the pathogenic bacteria Listeria monocytogenes and Salmonella berta from a Cumberland sausage. In all three phase systems, the food particles partitioned to the lower phase. Best performance was obtained by the polymer combinations, polyethylene glycol 3350/dextran T 500 and polyethylene glycol 3350/hydroxypropyl starch. In these systems, Salmonella berta partitioned to the hydrophobic upper phase both at pH 3 and pH 6 with an average partitioning ratio of 80% and a recovery of 56%. Listeria monocytogenes partitioned to the upper phase at pH 3 only with an average partitioning ratio of 72% and a recovery of 45%. This method may become a valuable tool for separation of bacteria from complex food matrices.