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Nucleotide sequence of the nifH gene coding for nitrogen reductase in the acetic acid bacterium Acetobacter diazotrophicus
Author(s) -
Franke,
Fegan,
Hayward,
Sly
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00258.x
Subject(s) - biology , gene , azospirillum brasilense , nucleic acid sequence , genetics , phylogenetic tree , polymerase chain reaction , sequence analysis , bacteria , nitrogen fixation
The nifH gene sequence of the nitrogen‐fixing bacterium Acetobacter diazotrophicus was determined with the use of the polymerase chain reaction and universal degenerate oligonucleotide primers. The gene shows highest pair‐wise similarity to the nifH gene of Azospirillum brasilense . The phylogenetic relationships of the nifH gene sequences were compared with those inferred from 16S rRNA gene sequences. Knowledge of the sequence of the nifH gene contributes to the growing database of nifH gene sequences, and will allow the detection of Acet. diazotrophicus from environmental samples with nifH gene‐based primers.