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A mimic as internal standard to monitor PCR analysis of food‐borne pathogens
Author(s) -
Lambertz S. Thisted
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00256.x
Subject(s) - yersinia enterocolitica , biology , microbiology and biotechnology , enterobacteriaceae , bacteria , chromatography , chemistry , escherichia coli , gene , genetics
This report describes a method for the construction of a mimic molecule for monitoring PCR. A blue cheese homogenate spiked with Yersinia enterocolitica was used to demonstrate how false‐negative PCR results may be avoided using the mimic. Further, the possibility of estimating bacterial numbers using the mimic was illustrated by varying the target concentration.