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Immunomagnetic separation for enhanced flagellar antigen phase inversion in salmonella
Author(s) -
Davies R. H.,
Wray C.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00390.x
Subject(s) - immunomagnetic separation , salmonella , antigen , bacteria , biology , microbiology and biotechnology , chemistry , chromatography , immunology , genetics
Immunomagnetic separation (IMS) was used to reduce delays in serotyping caused by slow flagellar phase inversion of diphasic salmonellas. Some 375 strains of 11 salmonella serotypes were examined using IMS. The mean time for successful flagellar phase inversion was reduced from 4·3 (range 1–18) d to 1·0 (range 1–3) d using the IMS method, and in a further experiment phase inversion was achieved within 8 h in 112 of a further 117 strains representing four salmonella serotypes.