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Optimization of RAPD for fingerprinting Salmonella
Author(s) -
Hilton A. C.,
Banks J. G.,
Penn C. W.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00385.x
Subject(s) - rapd , salmonella , dna profiling , biology , microbiology and biotechnology , genetics , bacteria , dna , genetic diversity , medicine , environmental health , population
Random amplification of polymorphic DNA (RAPD) is proving to be a useful technique in studying the epidemiology of micro‐organisms. The technique can be troublesome and time consuming to establish due to the essentially empirical approach to optimization. By standardization of certain parameters and use of a commercially available PCR buffer optimization kit, a particularly promising primer was identified and RAPD conditions for a highly discriminatory and reproducible characterization of Salmonella isolates was achieved. In addition, a technique to obtain reproducible RAPD fingerprints of Salmonella isolates without the need to purify genomic DNA is described.