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Use of oxygen‐permeable silicone rubber pouches for growing mass cultures of bacteria
Author(s) -
Giambernardi T. A.,
Klebe R. J.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00381.x
Subject(s) - laboratory flask , silicone rubber , bacteria , oxygen permeability , oxygen , bacterial growth , natural rubber , shaker , cuvette , silicone , chemistry , microbiology and biotechnology , biology , organic chemistry , quantum mechanics , vibration , genetics , physics
Growth of most bacteria often involves the use of expensive incubated shaker systems. In this report, oxygen‐permeable silicone rubber pouches, with oxygen permeability over 100 times higher than other polymers, were employed for growing bacterial cultures. With little, if any, agitation oxygen‐permeable silicone rubber pouches produced bacterial growth rates equivalent to growth rates obtained in shaker flasks. The silicone rubber pouch described has a glass cuvette integrated into its design that permits readings of bacterial density without opening the pouch. One can sterilize and store powdered bacterial culture medium in silicone rubber pouches ; therefore, bacterial cultures can be initiated by simply adding water and bacteria.