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A simple artificial urine for the growth of urinary pathogens
Author(s) -
Brooks T.,
Keevil C.W.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00378.x
Subject(s) - urine , urinary system , liquid medium , agar , urease , microbiology and biotechnology , biology , agar plate , chromatography , chemistry , bacteria , urea , biochemistry , endocrinology , genetics
A simple artificial urine medium (AUM) has been developed which provides conditions similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide range of urease‐positive and ‐negative urinary pathogens. Liquid AUM supported growth at concentrations of up to 10 8 cfu ml −1 , as found in normal urine. Reproducible, steady‐state growth also occurred over many generations in continuous culture. AUM was capable of forming crystals and encrustations resembling those found in natural urinary tract infections. The medium is a suitable replacement for normal urine for use in a wide range of experiments modelling the growth and attachment of urinary pathogens in the clinical environment.