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Isolation of antibiotic‐sensitive mutants of Azotobacter chroococcum by treatment with ascorbic acid
Author(s) -
Garg F. C.,
Bharati R.,
Sharma P. K.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00369.x
Subject(s) - azotobacter chroococcum , ascorbic acid , mutant , microbiology and biotechnology , novobiocin , penicillin , antibiotics , nalidixic acid , biology , biochemistry , strain (injury) , chemistry , bacteria , antibiotic resistance , gene , food science , genetics , anatomy
A mutant of Azotobacter chroococcum sensitive to penicillin and novobiocin was isolated by treatment with 2 m mol l −1 ascorbic acid. Presence of ascorbic acid was found to stimulate the inhibitory effect of both the antibiotics in A. chroococcum Mac 27 and its mutant Mac 27 (671) ; however, the effect was more pronounced in mutant Mac 27 (671). The mutant strain Mac 27 (671) did not produce β‐lactamase, a property which was present in the strain Mac 27.