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Detection of Salmonella spp. in food products by polymerase chain reaction and hybridization assay in microplate format
Author(s) -
Soumet C.,
Ermel G.,
Salvat G.,
Colin P.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00358.x
Subject(s) - salmonella , polymerase chain reaction , agarose , dna , microbiology and biotechnology , chemistry , biology , hybridization probe , dna–dna hybridization , chromatography , bacteria , food science , biochemistry , gene , genetics
Here, hybridization assay of amplified products is described which detect Salmonella sp. from chicken fillets and other food homogenates within 24 h. This technique is composed of four steps : (1) sample is pre‐enriched overnight in phosphate buffered peptone water ; (2) total DNA is extracted ; (3) a Salmonella spp. specific DNA target sequence is amplified by polymerase chain reaction ; (4) amplified products are captured by a probe covalently bound onto NH‐Covalink TM (Nunc, Danemark) microwells and detected by a chemiluminescent enzymatic reaction. This hybridization of amplified products was demonstrated as sensitive as their analysis on agarose gel. Compared to a bacteriological method for Salmonella spp. detection, its specificity was estimated at 100% and its sensitivity was 93·2% from analysis of 207 naturally contaminated chicken fillets samples.