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Factors affecting the production of an antimicrobial agent, plantaricin F, by Lactobacillus plantarum BF001
Author(s) -
Paynter M. J. B.,
Brown K. A.,
Hayasaka S. S.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00356.x
Subject(s) - fermentation , food science , lactobacillus plantarum , lactobacillaceae , bacterial growth , bacteria , biology , metabolite , lactobacillus , antimicrobial , chemistry , lactic acid , microbiology and biotechnology , biochemistry , genetics
Lactobacillus plantarum BF001 produced plantaricin F in MRS broth but it was detected only after ca a 50‐fold concentration. Growth on MRS broth and appearance of plantaricin F were similar under aerobic and anaerobic conditions. No growth occurred at pH 3 or at 4°C. Plantaricin F appeared first at early stationary growth phase (24 h) and was stable thereafter (pH 2). Amounts found in liquid cultures were ca 2–3 times higher than those from solidified MRS medium, and specific activities were ca 6 times higher in liquid culture (48 h). Maximal amounts of plantaricin F were found (48 h) when medium had an initial pH of 4 and growth was at 30°C. Under these conditions, cell growth and fermentation were partially uncoupled. Plantaricin F was not produced endogenously, organic nutrients were necessary. A molecular weight range of 500–3500 Da was indicated. Plantaricin F appears to be a secondary metabolite.